Structural analysis of arabinose-5-phosphate isomerase fromBacteroides fragilisand functional implications
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چکیده
منابع مشابه
Analysis of the arabinose-5-phosphate isomerase of Bacteroides fragilis provides insight into regulation of single-domain arabinose phosphate isomerases.
Arabinose-5-phosphate isomerases (APIs) catalyze the interconversion of d-ribulose-5-phosphate and D-arabinose-5-phosphate, the first step in the biosynthesis of 3-deoxy-D-manno-octulosonic acid (Kdo), an essential component of the lipopolysaccharide in Gram-negative bacteria. Classical APIs, such as Escherichia coli KdsD, contain a sugar isomerase domain and a tandem cystathionine beta-synthas...
متن کاملEscherichia coli YrbH is a D-arabinose 5-phosphate isomerase.
A gene encoding for arabinose 5-phosphate isomerase (API), which catalyzes the interconversion of d-ribulose 5-phosphate (Ru5P) and d-arabinose 5-phosphate (A5P), has been identified from the genome of Escherichia coli K-12. API is the first enzyme in the biosynthesis of 3-deoxy-d-manno-octulosonate (KDO), a sugar moiety located in the lipopolysaccharide layer of most Gram-negative bacteria. Th...
متن کاملCharacterization of Escherichia coli D-arabinose 5-phosphate isomerase encoded by kpsF: implications for group 2 capsule biosynthesis.
In Escherichia coli, there are multiple paralogous copies of the enzyme API [A5P (D-arabinose 5-phosphate) isomerase], which catalyses the conversion of the pentose pathway intermediate Ru5P (D-ribulose 5-phosphate) into A5P. A5P is a precursor of Kdo (3-deoxy-D-manno-octulosonate), an integral carbohydrate component of various glycolipids coating the surface of the OM (outer membrane) of Gram-...
متن کاملIdentification of GutQ from Escherichia coli as a D-arabinose 5-phosphate isomerase.
The glucitol operon (gutAEBDMRQ) of Escherichia coli encodes a phosphoenolpyruvate:sugar phosphotransferase system that metabolizes the hexitol D-glucitol (sorbitol). The functions for all but the last gene, gutQ, have been previously assigned. The high sequence similarity between GutQ and KdsD, a D-arabinose 5-phosphate isomerase (API) from the 3-deoxy-D-manno-octulosonate (KDO)-lipopolysaccha...
متن کاملL-arabinose Negative Mutants of the L-ribulokinase Structural Gene Affecting the Levels of L-arabinose Isomerase in Escherichia Coli.
C @ 51 37 19 12 5 42 3 49 57 21 59 1.U 1 1 1 1 l 1 1 1 WENTY-two L-arabinose nonutilizing mutants of Eschrichia coli B/r have Tbeen ordered with regard to each other and a closely linked leucine marker by three-point tests using transduction of E . coli by phage Plbt (GROSS and ENGLESBERG 1959; ENGLESBERG, ANDERSON, WEINBERG, LEE, HOFFEE, HUTTENHAUER and BOYER 1962; BOYER, ENGLESBERG and WEINBE...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section D Biological Crystallography
سال: 2014
ISSN: 1399-0047
DOI: 10.1107/s1399004714017052